Description

The qScript™ cDNA Synthesis Kit is a sensitive and easy-to-use solution for RNA quantification using two step RT-PCR. The novel qScript™ Reaction Mix provides all the necessary components for cDNA synthesis except enzyme and RNA template. The optimized blend of random and oligo(dT) primers provides robust, consistent and unbiased first-strand synthesis over a broad range of RNA template concentrations. qScript™ reverse transcriptase is a mixture of an engineered MMLV RT and a ribonuclease inhibitor protein. The simplified reaction procedure is ideally suited for high throughput expression studies using real time quantitative RT-PCR. The resulting cDNA product is directly compatible with current real-time PCR methods or conventional end-point RT-PCR of targets ≤1kb in length.

Features & Benefits

• High sensitivity – optimised for two-step quantitative RT-PCR
• Broad dynamic range
• Unbiased cDNA synthesis – complete 5′ to 3′ RNA sequence representation
• Easy reaction assembly – SuperMix format uses just two tubes

Components

• qScript Reaction Mix (5X)
  5X concentrated solution of optimized buffer, magnesium, primers (oligo(dT)20 + random primers), and dNTPs
• qScript Reverse Transcriptase, 20X concentration
• Nuclease-free water

Shipping & Storage

Kit components are stable for one year when stored in a constant temperature freezer at -20°C.  After thawing, mix thoroughly before using

Protocol PDF