Description

5x HOT FIREPol^® Probe Universal qPCR Mix is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables efficient amplification of AT-rich, regular and GC-rich (up to 75%) targets. HOT FIREPol^® Probe Universal qPCR Mix is optimized for DNA/LNA hydrolysis probes based on the 5´–3´ exonuclease activity.

HOT FIREPol^® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Applications

• Detection and quantification of DNA and cDNA targets
• Profiling gene expression
• Microbial detection
• Viral load determination

Features & Benefits

• Increased sensitivity and specificity for wide range of templates, including AT-rich, regular and GC-rich cDNA and gDNA
• Suitable for singleplex and duplex assays
• Reaction set-up at room temperature -master mix is stable at ambient temperature for one month
• Mix contains dUTP – it may be used with UNG to prevent carryover contamination from previous qPCR runs (UNG treatment)
• Use with any hydrolysis probe (LNA or DNA)
• Wide instrument compatibility: the mix is compatible with any real-time instrument other then capillary

Composition

• HOT FIREPol^® DNA Polymerase
• 5x Probe Universal qPCR buffer
• 15mM MgCl₂ 1x PCR solution – 3mM
• dNTPs blend containing dUTP/dTTP
• Internal reference based on ROX dye

Shipping & Storage

Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of  5x HOT FIREPol^® Probe Universal qPCR Mix.

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