dnature diagnostics & research

PerfeCTa® PreAmp SuperMix, 40 x 50 µl reactions

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95146-040

SKU: 95146-040 Categories: , ,

Description

PerfeCTa PreAmp SuperMix is a 5x concentrated, ready-to-use reaction cocktail for unbiased, selected enrichment of target sequences from limiting amounts of starting material for downstream gene expression profiling or targeted re-sequencing. It contains all the necessary components, except primers and templates. The 5x concentrated SuperMix allows addition of higher template volumes when working with low concentration samples, and/or reduced reaction volumes. Inclusion of inert light blue tracer dye helps visualize small reaction volumes and ensure accurate pipetting. PerfeCTa PreAmp SuperMix delivers unbiased pre-amplification of up to 100.

Features & Benefits

  • Unbiased, linear pre-amplification of up to 100 DNA targets
  • 5x concentrated SuperMix maximizes sample input volume with dilute cDNA templates
  • Superior assay sensitivity and specificity with ultrapure AccuStart II enzyme technology – maximum-yielding Taq DNA polymerase mutant controlled by stringent, multi-epitope antibody hot-start
  • User-friendly inert AccuVue plate loading dye provides visual confirmation of reagent addition

Components

  • 5X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-startDNA polymerase, AccuVue™ blue tracer dye, and stabilizers.

Shipping & Storage

Store components in a constant temperature freezer at -25°C to -15°C protected from light upon receipt.
Repeated freezing and thawing does not impair product performance.

Protocol

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