PerfeCTa PreAmp SuperMix is a 5x concentrated, ready-to-use reaction cocktail for unbiased, selected enrichment of target sequences from limiting amounts of starting material for downstream gene expression profiling or targeted re-sequencing. It contains all the necessary components, except primers and templates. The 5x concentrated SuperMix allows addition of higher template volumes when working with low concentration samples, and/or reduced reaction volumes. Inclusion of inert light blue tracer dye helps visualize small reaction volumes and ensure accurate pipetting. PerfeCTa PreAmp SuperMix delivers unbiased pre-amplification of up to 100.
Features & Benefits
- Unbiased, linear pre-amplification of up to 100 DNA targets
- 5x concentrated SuperMix maximizes sample input volume with dilute cDNA templates
- Superior assay sensitivity and specificity with ultrapure AccuStart II enzyme technology – maximum-yielding Taq DNA polymerase mutant controlled by stringent, multi-epitope antibody hot-start
- User-friendly inert AccuVue plate loading dye provides visual confirmation of reagent addition
- 5X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-startDNA polymerase, AccuVue™ blue tracer dye, and stabilizers.
Shipping & Storage
Store components in a constant temperature freezer at -25°C to -15°C protected from light upon receipt.
Repeated freezing and thawing does not impair product performance.