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5x HOT FIREPol® EvaGreen® qPCR Supermix, 1 ml

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08-36-00001 – 5x HOT FIREPol® EvaGreen® qPCR Supermix

Description

A 5X optimised ready-to-use solution for dye-based real-time quantitative PCR assays. This master mix has been developed to give highly specific and sensitive results and demonstrates excellent performance with GC-rich regions.  A mix for dye-based qPCR detection and quantitation of target DNA sequences using EvaGreen® dye and SYBR®/FAM channel of most real-time qPCR instruments.

The Mix contains HOT FIREPol® DNA Polymerase and a passive internal reference dye that is compatible with most qPCR cyclers including no ROX and low ROX* reference signal requiring platforms.

Features & Benefits

  • high sensitivity with low DNA concentrations
  • blue dye to ease pipetting
  • reduced primer dimer formation
  • one qPCR mix for all cyclers (except capillary and high ROX* cyclers)
  • contains dUTP to prevent cross-contamination with UNG treatment

Applications

  • detection and quantification of DNA and cDNA targets
  • profiling gene expression
  • microbial detection
  • viral load determination

Mix Components

  • HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start
  • 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl21x PCR solution – 2.5 mM MgCl2
  • dNTPs, including dUTP: mix allows UNG treatment to prevent carryover contamination from previous runs.
    IMPORTANT: UNG is not included in the 5x HOT FIREPol® EvaGreen® Supermix
  • EvaGreen® dye
  • Internal reference based on ROX dye
  • GC-enhancer
  • Blue visualisation dye

Shipping & Storage

Routine storage: -20oC

Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® EvaGreen® qPCR Supermix.

 

* IMPORTANT UPDATE! HOT FIREPol® EvaGreen® qPCR Supermix is not compatible with high ROX cyclers such as Applied BioSystems® StepOne™ or StepOnePlus™

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