Description

A 5X optimised ready-to-use solution for dye-based real-time quantitative PCR assays. This master mix has been developed to give highly specific and sensitive results and demonstrates excellent performance with GC-rich regions.  A mix for dye-based qPCR detection and quantitation of target DNA sequences using EvaGreen^® dye and SYBR^®/FAM channel of most real-time qPCR instruments.

Features & Benefits

• high sensitivity with low DNA concentrations
• blue dye to ease pipetting
• reduced primer dimer formation
• one qPCR mix for all cyclers (except capillary)
• contains dUTP to prevent cross-contamination with UNG treatment

Applications

• Detection and quantification of DNA and cDNA targets
• Profiling gene expression
• Microbial detection
• Viral load determination

Mix composition

• HOT FIREPol^® DNA polymerase: chemically modified FIREPol^® DNA Polymerase enabling hot-start
• 5x EvaGreen^® qPCR buffer with 12. 5 mM MgCl₂: 1x PCR solution – 2.5 mM MgCl₂
• dNTPs, including dUTP: mix allows UNG treatment to prevent carryover contamination from previous runs.
  IMPORTANT: UNG is not included in the 5x HOT FIREPol^® EvaGreen^® Supermix
• EvaGreen^® dye
• Internal reference based on ROX dye
• GC-enhancer
• Blue visualisation dye

Shipping & Storage

Typically store at -20^oC but can be shipped at room temperature and is shelf-stable at room temperature for one month.

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