Description

A 5X optimised ready-to-use solution for dye-based real-time quantitative PCR assays. This master mix has been developed to give highly specific and sensitive results and demonstrates excellent performance with GC-rich regions.  A mix for dye-based qPCR detection and quantitation of target DNA sequences using EvaGreen^® dye and SYBR^®/FAM channel of most real-time qPCR instruments.

The Mix contains HOT FIREPol^® DNA Polymerase and a passive internal reference dye that is compatible with most qPCR cyclers including no ROX and low ROX* reference signal requiring platforms.

Features & Benefits

• high sensitivity with low DNA concentrations
• blue dye to ease pipetting
• reduced primer dimer formation
• one qPCR mix for all cyclers (except capillary and high ROX* cyclers)
• contains dUTP to prevent cross-contamination with UNG treatment

Applications

• detection and quantification of DNA and cDNA targets
• profiling gene expression
• microbial detection
• viral load determination

Mix Components

• HOT FIREPol^® DNA polymerase: chemically modified FIREPol^® DNA Polymerase enabling hot-start
• 5x EvaGreen^® qPCR buffer with 12. 5 mM MgCl₂: 1x PCR solution – 2.5 mM MgCl₂
• dNTPs, including dUTP: mix allows UNG treatment to prevent carryover contamination from previous runs.
  IMPORTANT: UNG is not included in the 5x HOT FIREPol^® EvaGreen^® Supermix
• EvaGreen^® dye
• Internal reference based on ROX dye
• GC-enhancer
• Blue visualisation dye

Shipping & Storage

Routine storage: -20^oC

Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol^® EvaGreen^® qPCR Supermix.

* IMPORTANT UPDATE! HOT FIREPol^® EvaGreen^® qPCR Supermix is not compatible with high ROX cyclers such as Applied BioSystems^® StepOne™ or StepOnePlus™