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Ultraplex™ 1-Step ToughMix, Low ROX, 100 reactions

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95168-100 – Ultraplex™ 1-Step ToughMix, Low ROX, 100 reactions

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Description

UltraPlex 1-Step ToughMix is a ready-to-use, 4X concentrated master mix 1-step reverse transcription and real-time quantitative PCR (RT-qPCR) of RNA templates using probe-based detection methods. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between steps. Optimized to deliver highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or highly multiplexed RNA detection assays, this reagent chemistry is optimized to deliver maximum assay sensitivity, precision and reproducibility with miniaturized reaction volumes and either conventional or accelerated thermal cycling protocols. UltraPlex 1-Step ToughMix contains all required components for RT-qPCR except RNA template and probe and is compatible with all dual-labeled probe chemistries.

Features & Benefits

  • 4X concentrated master mix allows greater flexibility with dilute samples – as little as 1 pg of total RNA
  • Multiplexing capable with up to 5 targets
  • Tough-tested for applications where PCR inhibition from template impurities may be a concern, such as: crude extracts, clinical specimens, or environmental samples.
  • Temperature stabilized master mix enables convenient reaction setup at ambient room temperature

Contents

UltraPlex 1-Step ToughMix (Low ROX/ ROX) (4X), contains:

  • 4X reaction buffer containing optimized titrations of qPCR-grade dATP, dCTP, dGTP, dTTP, and magnesium.
  • Ultra-pure recombinant proteins: qScript XLT reverse transcriptase, RNase inhibitor protein, and AccuStart II hot-start Taq DNA polymerase.
  • Proprietary stabilizers additives and optimized ROX passive reference dye (if applicable).

Storage & Handling

Store components in a constant temperature freezer at -25°C to -15°C protected from light upon receipt. For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.
Repeated freezing and thawing does not affect RT-qPCR performance.

Protocol

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