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Quantabio qScript® XLT One-Step RT-qPCR ToughMix® Low ROX, 100 x 20 µl reactions

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95134-100

SKU: 95134-100 Categories: , ,

Description

qScript® XLT One-Step RT-qPCR ToughMix® is a ready-to-use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan® 5′-hydrolysis probes. First-strand cDNA synthesis and subsequent PCR amplification are carried out seamlessly in the same reaction mixture with optimized 1-step thermal cycling parameters. This kit is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets as well as high throughput gene-expression studies. The system has been optimized to deliver maximum RT-PCR efficiency, sensitivity, and specificity in minimal reaction volumes and accelerated thermal cycling rates. The only necessary user-supplied materials for RT-qPCR is RNA sample and probe assay. It is compatible with all types of molecular probe assays including dual-labeling strategies. Elevating cDNA synthesis reaction temperature to 50-55°C during one-step RT-qPCR improves primer annealing and disruption of RNA secondary structure that can interfere with cDNA synthesis.

Features & Benefits

  • ToughMix reagent technology neutralizes a broad spectrum of PCR inhibitors
  • Superior assay sensitivity and specificity with ultrapure AccuStart II enzyme technology
  • Inert AccuVue plate loading dye simplifies reaction setup and provides instant visual cue of reagent addition and mixing
  • Supports efficient vortex mixing with proprietary anti-foaming formulation
  • Flexible – supports for both fast and standard thermal cycling conditions

Components

2X reaction buffer containing

  • Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP
  • qScript XLT reverse transcriptase
  • RNase inhibitor protein
  • AccuStart II Taq DNA Polymerase
  • Inert AccuVue™ plate loading dye
  • Proprietary enzyme stabilizers and performance-enhancing additives
  • Titrated reference dye (if applicable)

Shipping & Storage

Store components in a constant temperature freezer at -25°C to -15°C protected from light upon receipt.
Repeated freezing and thawing does not affect RT-qPCR performance.

 

Application notes for COVID-19 testing

 

Protocol

Product Flyer

Application Note

 

E Gene Comparison between ToughMix

E Gene Comparison between ToughMix

E Gene comparing qScript 1-Step Virus ToughMix vs. qScript XLT 1-Step RT-qPCR ToughMix. 15, 150 and 1500 copies of RNA were compared. qScript 1-Step Virus ToughMix improves low copy RT-qPCR performance at both 150 and 15 copies of RNA.

 

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