dnature diagnostics & research

Quantabio qScript® 1-Step Virus ToughMix®, 100 x 20 µl reactions

Enquire for price

95131-100

Description

qScript® 1-Step Virus ToughMix® is a 2x, ready-to-use, master mix for rapid detection of RNA viruses such as Flu-A, Flu-B, and SARS-CoV-2, using one-step, or single-tube reverse transcription quantitative PCR (RT-qPCR). It has been optimized for maximum sensitivity to enable reliable quantification of very low input quantities of RNA using dual-labeled hydrolysis probe detection chemistries such as TaqMan® probes in single or multiplexed assay formats. qScript® 1-Step Virus ToughMix® contains all required components for RT-qPCR except RNA template and primer/probe assays.

 

qScript® 1-Step Virus ToughMix® is powered by an engineered reverse transcriptase with reduced RNase H activity and improved activity and stability at higher temperatures, that includes a RT Hot-Start technology to suppresses non-specific primer extension by the RT before cDNA synthesis. The use of higher temperatures (50 to 55°C) for the first-strand step of one-step RT-qPCR provides higher specificity for primer annealing and disruption of RNA secondary structure that can interfere with cDNA synthesis. These features combined with a stringent mAb hot-start Taq polymerase and ToughMix reagent technology to neutralize many common PCR inhibitors, provide reproducible low-copy quantification as well as extended room temperature stability of fully assembled reactions for automated reaction assembly.

qScript® 1-Step Virus ToughMix® does not contain an internal reference dye.

Features & Benefits

  • Superior Sensitivity – higher amplification yields enable lower copy detection
  • Tough Tested – tolerant to a wide range of PCR inhibitors
  • Multiplexing – highly sensitive detection for up to four targets

Components

2X reaction buffer containing

  • dATP, dCTP, dGTP, dTTP, magnesium chloride,
  • qScript XLT reverse transcriptase, RNase inhibitor protein, hot-start DNA polymerase,
  • AccuVue blue qPCR dye, and stabilizers

Shipping & Storage

Store components in a constant temperature freezer at -25°C to -15°C. Repeated freezing and thawing do not affect RT-qPCR performance.

Application notes for COVID-19 testing

Protocol

Product Flyer

Application Note

 

E Gene Comparison between ToughMix

E Gene Comparison between ToughMix

E Gene comparing qScript 1-Step Virus ToughMix vs. qScript XLT 1-Step RT-qPCR ToughMix. 15, 150 and 1500 copies of RNA were compared. qScript 1-Step Virus ToughMix improves low copy RT-qPCR performance at both 150 and 15 copies of RNA.

 

Be the first to review “Quantabio qScript® 1-Step Virus ToughMix®, 100 x 20 µl reactions”

Related Products