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qScript 1-Step Virus ToughMix, 2,000 reactions

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qScript 1-Step Virus ToughMix is a 2x, ready-to-use, master mix for rapid detection of RNA viruses such as Flu-A, Flu-B, and SARS-CoV-2, using one-step, or single-tube reverse transcription-quantitative PCR (RT-qPCR).

It has been optimised for maximum sensitivity to enable reliable quantification of very low input quantities of RNA using dual-labelled hydrolysis probe detection chemistries such as TaqMan® probes in single or multiplexed assay formats.  qScript 1-Step Virus ToughMix contains all required components for RT-qPCR except RNA template and primer/probe assays.

Features & Benefits

  • Superior Sensitivity – higher yields optimised for lower viral RNA inputs
  • Tough Tested – tolerant to a wide range of PCR inhibitors
  • Multiplexing – Supports highly sensitive detection for up to four targets
  • Broad Dynamic Range – Reliable data from your precious samples every time

Real-Time PCR Instrument Compatibility

  • Roche LightCycler™ 480
  • Qiagen/Corbett Rotor-Gene®
  • Illumina Eco
  • MIC
  • Eppendorf Mastercycler®
  • Cepheid SmartCycler®
  • Bio-Rad CFX96™
  • Bio-Rad CFX384™


2X reaction buffer containing dATP, dCTP, dGTP, dTTP, magnesium chloride, qScript XLT reverse transcriptase, RNase inhibitor protein, hot-start DNA polymerase, AccuVue blue qPCR dye, and stabilisers

Shipping & Storage

qScript XLT One-Step RT-qPCR ToughMix is stable for one year when stored in a constant temperature freezer at -20°C protected from light. Repeated freezing and thawing does not affect RT-qPCR performance.

PDF Protocol

Application notes for COVID-19 testing


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