qScript XLT One-Step RT-qPCR ToughMix is a ready-to-use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan® 5′-hydrolysis probes. First-strand cDNA synthesis and subsequent PCR amplification are carried out seamlessly in the same reaction mixture with optimized 1-step thermal cycling parameters. This kit is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets as well as high throughput gene-expression studies. The system has been optimized to deliver maximum RT-PCR efficiency, sensitivity, and specificity in minimal reaction volumes and accelerated thermal cycling rates. The only necessary user-supplied materials for RT-qPCR is RNA sample and probe assay. It is compatible with all types of molecular probe assays including dual-labeling strategies. Elevating cDNA synthesis reaction temperature to 50-55°C during one-step RT-qPCR improves primer annealing and disruption of RNA secondary structure that can interfere with cDNA synthesis.
Features & Benefits
- Tough Tested – Overcomes common inhibitors including polysacharides, heme/hemoglobin, humic acid, melanin
- Flexible – Use fast or standard qPCR cycling conditions
- Broad dynamic range- Reliable data from your precious samples every time
- Multiplexing enabled – Supports highly sensitive detection for up to four targets
2X concentrated One-Step Master Mix containing:
- Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP
- qScript XLT reverse transcriptase
- RNase inhibitor protein
- AccuStart II Taq DNA Polymerase
- Inert AccuVue™ plate loading dye
- Proprietary enzyme stabilizers and performance-enhancing additives
- Titrated reference dye (if applicable)
Storage & Shipping
qScript XLT One-Step RT-qPCR ToughMix is stable for up to one year when stored in a constant temperature freezer at -20°C protected from light. Repeated freezing and thawing does not affect RT-qPCR performance.