Description

FIREPol^® is a highly processive, thermostable DNA Polymerase. Due to its genetic modifications, it has enhanced stability at room temperature with no activity loss for up to 1 month.

Possesses 5′→3′ polymerase and 5′→3′ exonuclease activity, as well as a non-template-dependent terminal transferase activity, but lacks a 3′→5′ exonuclease (proofreading) activity making the generated product suitable for TA-cloning.

The fidelity of FIREPol^® is similar to a regular Taq DNA Polymerase (error rate per nucleotide ca 2.5 x10^-5)

Applications

• routine PCR
• Suited for a wide range of PCR assays
• TA cloning

Components

• FIREPol® DNA Polymerase
• 10 x Reaction buffer B (Mg²⁺ free)
  0.8 M Tris-HCl, 0.2 M (NH₄)₂SO₄, 0.2% w/v Tween-20
• 10 x Reaction buffer BD (Mg²⁺ and detergent free)
  0.8 M Tris-HCl, 0.2 M (NH₄)₂SO₄
• 10 x Solution S
  PCR additive for difficult templates (e.g. GC-rich DNA templates, high secondary structure)
• 25 mM MgCl₂

Dilution Buffer

50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Shipping & Storage

Routine storage: -20°C

Shipping and Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of FIREPol^® DNA Polymerase.