Description

FIREPol® is a highly processive, thermostable DNA polymerase. Due to its proprietary modifications FIREPol® has enhanced stability at room temperature with no activity loss for up to 1 month at R/T. The enzyme has 5’→3′ exonuclease activity but lacks 3’→ 5′ activity.

Applications

• routine PCR
• Suited for a wide range of PCR assays
• TA cloning

Components

• FIREPol® DNA Polymerase
• 10 x Reaction buffer B (Mg2+ free)
  0.8 M Tris-HCl, 0.2 M (NH4)2SO4, 0.2% w/v Tween-20
  
• 10 x Reaction buffer BD (Mg2+ and detergent free)
  0.8 M Tris-HCl, 0.2 M (NH4)2SO4
• 10 x Solution S
  PCR additive for difficult templates (e.g. GC-rich DNA templates, high secondary structure)

Dilution Buffer

50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Shipping & Storage

Routine storage: -20 ºC Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of FIREPol® DNA polymerase.

Protocol PDF