dnature diagnostics & research

FIREPol® DNA polymerase 1000 units

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01-01-01000

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Description

FIREPol® is a highly processive, thermostable DNA Polymerase. Due to its genetic modifications, it has enhanced stability at room temperature with no activity loss for up to 1 month. 

Possesses 5′→3′ polymerase and 5′→3′ exonuclease activity, as well as a non-template-dependent terminal transferase activity, but lacks a 3′→5′ exonuclease (proofreading) activity making the generated product suitable for TA-cloning. 

The fidelity of FIREPol® is similar to a regular Taq DNA Polymerase (error rate per nucleotide ca 2.5 x10-5) 

Applications

  • routine PCR
  • suited for a wide range of PCR assays
  • TA cloning

Components

  • FIREPol® DNA Polymerase
  • 10 x Reaction buffer B (Mg2+ free)
    0.8 M Tris-HCl, 0.2 M (NH4)2SO4, 0.2% w/v Tween-20  
  • 10 x Reaction buffer BD (Mg2+ and detergent free)
    0.8 M Tris-HCl, 0.2 M (NH4)2SO4.
  • 10 x Solution S
    PCR additive for difficult templates (e.g. GC-rich DNA templates, high secondary structure)
  • 25 mM MgCl2

Dilution Buffer

50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Shipping & Storage

Routine storage: -20°C

Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of FIREPol® DNA polymerase.

Protocol PDF

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