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5x HOT FIREPol SolisGreen qPCR mix, 1 ml

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08-46-00001 – 5X HOT FIREPol SolisGreen qPCR mix

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Description

New from Solis BioDyne, this Dye-based real-time quantitative PCR (qPCR) uses DNA binding dye to evaluate the DNA amplification process during PCR. HOT FIREPol® SolisGreen® qPCR Mix is an optimized ready-to-use solution for real-time quantitative PCR assays, incorporating SolisGreen® dye.

It comprises all the components necessary, excluding the template and primers, to perform highly sensitive qPCR. The user simply needs to add water, template, and primers.

HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

 

Features & Benefits

  • improved detection of low target concentrations
  • higher fluorescence level
  • reaction set-up and shipment without dry ice

Applications

  • detection and quantification of DNA and cDNA targets
  • profiling gene expression
  • microbial detection
  • viral load determination

Mix Components

  • HOT FIREPol® DNA Polymerase
  • 5x qPCR buffer with 12.5 mM MgCl2 
  • 1x PCR solution – 2.5 mM
  • dNTPs blend containing dUTP/dTTP
  • SolisGreen® dye
  • Internal reference based on ROX dye

Shipping & Storage

Routine storage: -20°C

Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product.

Protocol

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