5x HOT FIREPol® GC Master Mix is designed to provide highly specific high-yield amplification of GC-rich and other difficult templates.
The Master Mix is a ready-to-use solution containing all reagents required for PCR (except template, primers and water). Extra vials of 100% DMSO and 25 mM MgCl2 enable flexibility in reaction optimization.
Generated PCR products are compatible with TA cloning procedures as well as analysis by capillary electrophoresis (e.g. the Fragment Analyzer). They do not contain loading dyes
- Hot Start GC-rich PCR
- Fragment analysis
- TA cloning
- HOT FIREPol® DNA polymerase
- 5x HOT FIREPol® GC Buffer
- 7.5 mM MgCl2 1x PCR solution – 1.5 mM MgCl2
Shipping & Storage
Routine storage: -20°C
Shipping and temporary storage for up to 1 month at room temperature or storage for up to 6 months at 2-8°C has no detrimental effects on the quality of 5x HOT FIREPol® GC Master Mix
Amplification of various sized amplicons with GC content as stated in lane. HOT FIREPol GC Master Mix successfully amplified products containing up to 79% GC content!