Description
ATP-independent, non-catalytic thermostable ligase that catalyzes the intramolecular ligation (i.e. circularization) of ssDNA templates.
Features
- Intramolecular ligation of single-stranded DNA ends containing 5′-phosphates and 3′-hydroxyl groups
- ATP-independent, non-catalytic enzyme
- Circularizes ssDNA >15 bases long
- Compatible with high temperature ligation reactions due to thermostability of CircLigase™ II enzyme
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