dnature diagnostics & research

5x HOT FIREPol® Blend Master Mix Ready to Load, 10mM Mg Sample

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04-25-00120S

SKU: 04-25-00120S Categories: , , ,

Description

These master mixes contain  all the reagents required for PCR (except template, primers and water) as convenient 5X masters.

They also contain buffer needed for direct loading of PCR reactions onto agarose gels following amplification and two tracking dyes (blue and yellow) to follow electrophoresis progress.

No more spotting out loading buffer – no more cutting lengths of parafilm!

These mixes are available in any size option that works for you.  Please contact us  – orders@dnature.co.nz for pricing and size options.

5 x HOT FIREPol® Blend Master Mix Ready to Load is a premixed ready-to-use solution containing all the reagents required for highly sensitive PCR (except template, primers and water). The master also includes compounds and tracking dyes for immediate loading of reaction onto gels post-PCR.

HOT FIREPol® Blend Master Mix contains two carefully optimised enzymes – HOT FIREPol® DNA polymerase and a proofreading polymerase. This enzyme blend has both the 5’→ 3’ exonuclease activity as well as the 3’→ 5’ proofreading activity. HOT FIREPol® Blend Master Mix exhibits an increased fidelity (up to five fold) compared to standard polymerases

Applications

  • rapid assay set-up for reactions requiring high sensitivity and/or specificity

Mix Composition

  • HOT FIREPol® DNA polymerase
  • Proofreading enzyme
  • 5 x Blend Master Mix Buffer
  • MgCl2 (concentration dependent on catalogue number)
  • 2mM dNTPs of each, 1 x PCR solution – 200µM dATP, 200µM dCTP, 200µM dGTP and 200µM dTTP
  • PCR adjuvants
  • Blue dye, Migration equivalent to 3.5-4.5kb DNA fragment
  • Yellow dye, Migration rate in excess of primers in 1% agarose gel: <35-45bp

1ml = 250 x 20µl reaction, 20ml = 5000 reactions and 100ml = 25,000 reactions

Shipping & Storage

Routine storage: -20ºC

Shipping and temporary storage for up to 1 month at room temperature or storage for up to 6 months at 2-8ºC has no detrimental effects on the quality of 5 x HOT FIREPol® Blend Master Mix, Ready to Load.

 

Protocol PDF

Reviews

  • Solis Biodyne HotStart FirePol is a great price/performance Taq enzyme. We get consistent great results with a product that is extremely easy to use. I’ve found it to be a very forgiving enzyme, it will amplify from less than stellar samples and tolerate the vagaries of rookie pipetting – KG, Massey University

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