Description

5x HOT FIREPol^® EvaGreen^® HRM Mix is an optimised ready-to-use solution for High Resolution Melt (HRM) Analysis, incorporating EvaGreen^® dye. It comprises all the components necessary to perform qPCR and HRM Analysis: HOT FIREPol^® DNA Polymerase, ultrapure dNTPs, MgCl₂ and EvaGreen^® dye. The user simply needs to add water, template and primers.

HOT FIREPol^® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Applications

• High Resolution Melt (HRM)

Components

• HOT FIREPol® DNA Polymerase
• 5x EvaGreen® HRM buffer
• 12.5 mM MgCl2
• dNTPs
• EvaGreen® dye
• No ROX dye

Shipping and Storage

Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol^® EvaGreen^® HRM Mix.

Protocol