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5x HOT FIREPol® EvaGreen® HRM Mix, 1 ml

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08-31-00001

SKU: 08-31-00001 Category:

Description

High-Resolution Melt (HRM) analysis is a fast and cost-effective method for post-PCR analysis to detect single nucleotide polymorphisms (SNP genotyping), genetic mutations, and DNA sequence variations.

5x HOT FIREPol® EvaGreen® HRM Mix is an optimised ready-to-use solution for High Resolution Melt (HRM) Analysis, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR and HRM Analysis: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2 and EvaGreen® dye. The user simply needs to add water, template and primers.

HOT FIREPol® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Features & Benefits

  • highly sensitive mix for accurate SNP detection
  • sensitive EvaGreen® dye for reliable HRM performance
  • reaction set-up without  ice

Applications

  • High Resolution Melt (HRM)
  • SNP detection
  • dye-based qPCR

Mix Components

  • HOT FIREPol® DNA Polymerase
  • 5x EvaGreen® HRM buffer
  • 12.5 mM MgCl, 1x PCR solution – 2.5 mM MgCl2 
  • dNTPs
  • EvaGreen® dye
  • No ROX dye

Shipping & Storage

Routine storage: -20oC

Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® EvaGreen® qPCR Supermix.

Protocol

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