Description

High-Resolution Melt (HRM) analysis is a fast and cost-effective method for post-PCR analysis to detect single nucleotide polymorphisms (SNP genotyping), genetic mutations, and DNA sequence variations.

5x HOT FIREPol^® EvaGreen^® HRM Mix is an optimised ready-to-use solution for High Resolution Melt (HRM) Analysis, incorporating EvaGreen^® dye. It comprises all the components necessary to perform qPCR and HRM Analysis: HOT FIREPol^® DNA Polymerase, ultrapure dNTPs, MgCl₂ and EvaGreen^® dye. The user simply needs to add water, template and primers.

HOT FIREPol^® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Features & Benefits

• highly sensitive mix for accurate SNP detection
• sensitive EvaGreen^® dye for reliable HRM performance
• reaction set-up without  ice

Applications

• High Resolution Melt (HRM)
• SNP detection
• dye-based qPCR

Mix Components

• HOT FIREPol® DNA Polymerase
• 5x EvaGreen® HRM buffer
• 12.5 mM MgCl, 1x PCR solution – 2.5 mM MgCl₂ 
• dNTPs
• EvaGreen® dye
• No ROX dye

Shipping & Storage

Routine storage: -20^oC

Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol^® EvaGreen^® qPCR Supermix.