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5x HOT FIREPol® EvaGreen® HRM Mix, 1ml

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08-31-00001

SKU: 08-31-00001 Category:

Description

5x HOT FIREPol® EvaGreen® HRM Mix is an optimised ready-to-use solution for High Resolution Melt (HRM) Analysis, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR and HRM Analysis: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2 and EvaGreen® dye. The user simply needs to add water, template and primers.

HOT FIREPol® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Applications

  • High Resolution Melt (HRM)

Components

  • HOT FIREPol® DNA Polymerase
  • 5x EvaGreen® HRM buffer
  • 12.5 mM MgCl2
  • dNTPs
  • EvaGreen® dye
  • No ROX dye

Shipping and Storage

Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® EvaGreen® HRM Mix.

Protocol

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