High-Resolution Melt (HRM) analysis is a fast and cost-effective method for post-PCR analysis to detect single nucleotide polymorphisms (SNP genotyping), genetic mutations, and DNA sequence variations.
5x HOT FIREPol® EvaGreen® HRM Mix is an optimised ready-to-use solution for High Resolution Melt (HRM) Analysis, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR and HRM Analysis: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2 and EvaGreen® dye. The user simply needs to add water, template and primers.
HOT FIREPol® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
Features & Benefits
- highly sensitive mix for accurate SNP detection
- sensitive EvaGreen® dye for reliable HRM performance
- reaction set-up without ice
- High Resolution Melt (HRM)
- SNP detection
- dye-based qPCR
- HOT FIREPol® DNA Polymerase
- 5x EvaGreen® HRM buffer
- 12.5 mM MgCl, 1x PCR solution – 2.5 mM MgCl2
- EvaGreen® dye
- No ROX dye
Shipping & Storage
Routine storage: -20oC
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® EvaGreen® qPCR Supermix.