Description
5x HOT FIREPol® EvaGreen® HRM Mix is an optimised ready-to-use solution for High Resolution Melt (HRM) Analysis, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR and HRM Analysis: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2 and EvaGreen® dye. The user simply needs to add water, template and primers.
HOT FIREPol® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
Applications
- High Resolution Melt (HRM)
Components
- HOT FIREPol® DNA Polymerase
- 5x EvaGreen® HRM buffer
- 12.5 mM MgCl2
- dNTPs
- EvaGreen® dye
- No ROX dye
Shipping and Storage
Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® EvaGreen® HRM Mix.
Be the first to review “5x HOT FIREPol® EvaGreen® HRM Mix, 1ml”
You must be logged in to post a comment.