The Steady-Luc™ HTS assay system is a homogeneous, high-sensitivity firefly luciferase reporter gene assay kit. It is a glow-type assay with a signal half-life of about 3 hours, allowing batch processing in microplates.
Firefly luciferase is widely used as a reporter for studying gene regulation and function and pharmaceutical screening.
It is a very sensitive genetic reporter due to the lack of endogenous activity in mammalian cells or tissues. However, the light production resulting from the reaction leads to the formation of suicidal adenyl-oxyluciferin at the enzyme surface. It results in a very short half-life of the light emission with flash-type kinetics. Because of this, flash-type luminescence must be detected immediately after initiating the reaction, which is not compatible with batch analysis of samples.
Biotium’s Steady-Luc™ Firefly Assay Buffer contains a proprietary mixture of substances that prevent the adenyl-oxyluciferin reaction, producing a long-lasting, glow-type signal. It produces a luminescence signal with a half-life of about 3 hours, for a high-throughput microplate assay.
Glow-type luciferase assays like Steady-Luc™ have lower luminescence signal compared to flash-type assays. The sensitivity and limit of detection of the assay will depend on luciferase expression levels in your experimental system as well as luminometer sensitivity.
- Simple, single-step homogeneous assay
- Luminescence half-life of about 3 hours
- Steady-Luc™ Firefly Assay Buffer
Delivery & Storage
- Delivered at room temperature but store at -20°C.
Available in a variety of pack sizes.