FIREPol® is a highly processive, thermostable DNA polymerase. Due to its proprietary modifications FIREPol® has enhanced stability at room temperature with no activity loss for up to 1 month at R/T. The enzyme has 5’→3′ exonuclease activity but lacks 3’→ 5′ activity.
- routine PCR
- Suited for a wide range of PCR assays
- TA cloning
- FIREPol® DNA Polymerase
- 10 x Reaction buffer B (Mg2+ free)
0.8 M Tris-HCl, 0.2 M (NH4)2SO4, 0.2% w/v Tween-20
- 10 x Reaction buffer BD (Mg2+ and detergent free)
0.8 M Tris-HCl, 0.2 M (NH4)2SO4
- 10 x Solution S
PCR additive for difficult templates (e.g. GC-rich DNA templates, high secondary structure)
50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.
Shipping & Storage
Routine storage: -20 ºC Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of FIREPol® DNA polymerase.