dnature diagnostics & research

FIREPol® DNA polymerase 20000 units

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01-01-20000

SKU: 01-01-20000 Categories: , ,

Description

FIREPol® is a highly processive, thermostable DNA polymerase. Due to its proprietary modifications FIREPol® has enhanced stability at room temperature with no activity loss for up to 1 month at R/T. The enzyme has 5’→3′ exonuclease activity but lacks 3’→ 5′ activity.

Applications

  • routine PCR
  • Suited for a wide range of PCR assays
  • TA cloning

Components

  • FIREPol® DNA Polymerase
  • 10 x Reaction buffer B (Mg2+ free)
    0.8 M Tris-HCl, 0.2 M (NH4)2SO4, 0.2% w/v Tween-20
  • 10 x Reaction buffer BD (Mg2+ and detergent free)
    0.8 M Tris-HCl, 0.2 M (NH4)2SO4
  • 10 x Solution S
    PCR additive for difficult templates (e.g. GC-rich DNA templates, high secondary structure)

Dilution Buffer

50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Shipping & Storage

Routine storage: -20 ºC Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of FIREPol® DNA polymerase.

Protocol PDF