Description

The Viral Nucleic Acid Extraction Kit II was designed specifically for efficient purification of viral DNA and viral RNA from cell-free samples such as serum, plasma, body fluids and the supernatant of viral infected cell cultures. The efficient glass fiber spin column system is optimized for nucleic acid purification from a wide variety of both DNA and RNA viruses such as HBV, CMV, HCV, HIV, and HTLV. 10¹-10⁹ copies of viral DNA/RNA can be purified from up to 200 µl samples within 20 minutes. The purified viral DNA/RNA can be used directly in qPCR and qRT-PCR assays.

Features & Benefits

• High Sensitivity – virus RNA/DNA can be successfully extracted and detected from as low as 10E1 copy number
• Purify virus DNA or virus RNA in 20 minutes
• Sample Volume –  up to 200 µl samples of plasma, serum, body fluids, supernatant of viral cell cultures
• Spin Columns –  glass fibre membrane optimized for virus DNA and virus RNA purification
• Individually packaged virus spin columns and collection tubes, certified RNase and DNase-free
• Elution Volume of 50 µl

Contents

• VB Lysis Buffer
• AD Buffer
• W1 Buffer
• Wash Buffer
• RNase-free Water
• VB Columns
• 2 ml Collection Tubes

Storage

Dry at room temperature 15-25 deg. C

Protocol PDF