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Presto™ Midi Plasmid Kit (Endotoxin Free) 25 Preps

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PIFE25

Description

The Presto™ Midi Plasmid Kit Endotoxin Freewas designed for Plasmid DNA Purification and endotoxin removal from 50-150 ml of cultured bacterial cells using an efficient plasmid midiprep system. For processing smaller volumes, the Presto™ Mini Plasmid Kit is also available. Both plasmid kits include TrueBlue Lysis Buffer (an optional color indicator) to prevent common handling errors, while ensuring efficient cell lysis and neutralization. The Presto™ Midi Plasmid Kit Endotoxin Free uses a modified alkaline lysis method and RNase treatment  to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. Using an efficient gravity-flow procedure, plasmid DNA in the crude lysate is bound by the plasmid midi column and the contaminants are removed with PW Buffer. The purified plasmid DNA is eluted by a high salt buffer and then precipitated with Isopropanol for desalting. The entire procedure can be completed in 80 minutes without ultracentrifuges, HPLC or other toxic reagents.

Features & Benefits

  • High Yield: >400 µg of pure transfection grade plasmid DNA from 100 ml of cultured bacterial cells
  • Efficient endotoxin removal (<0.1 EU/µg DNA) verified by LAL
  • Convenient: TrueBlue Lysis Buffer is an optional color indicator included with this plasmid kit in order to reduce common handling errors, while ensuring efficient cell lysis and neutralization and maximum plasmid product yield so you can feel confident with each and every plasmid prep
  • Sample Volume: 50-150 ml of cultured bacterial cells
  • Format: Plasmid Midi Anion-Exchange Resin Columns and Filter Columns (membranes optimized for plasmid DNA purification)
  • Elution Volume: 500 µl – 2 ml
  • Operation time: within 80 minutes (only 40 minutes when combined with the Presto™ Plasmid Concentrator)

 

Shipping & Storage

Dry store at room temperature (15-25°C) for up to two years

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