Description

The Magnetic Beads Genomic DNA Extraction Kit Bacteria was designed specifically for efficient genomic DNA purification from Gram (-) negative and Gram (+) positve bacteria. DNA is bound to the surface of the magnetic beads and released using a proprietary buffer system. The kit can be easily adapted to automated magnetic bead separation instruments and workstations. The purified DNA can be used in qPCR and a variety of other downstream applications.

Features & Benefits

• High Yield: >26 µg of Genomic DNA
• High Quality DNA: A260/A280 = 1.8-2.0
• Magnetic Bead Concentration: 50 mg/ml
• Magnetic Bead Size: ~ 5 µm
• Easily adapted to automated magnetic bead separation instruments and workstations
• Sample: 2 x 10⁹ of bacteria cells (Gram + and Gram -)
• Manual or automated DNA isolation
• Operation time: within 30 minutes (manual)

Applications

Automated Fluorescent DNA Sequencing, Southern Blotting, Next Generation Sequencing (NGS)

Contents

• ME1 Buffer
• ME2 Buffer
• ME3 Buffer
• ME4 Buffer
• Lysozyme
• Proteinase K
• MW1 Buffer
• MW2 Buffer
• ME Magnetic Beads
• Elution Buffer

Storage

Dry at room temperature (15-25ºC). Lysozyme is shipped at room temperature and should be stored at -20°C for extended periods

Protocol PDF