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TriRNA Pure Kit 50 Preps

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TRP050 – TriRNA Pure Kit 50 Preps

SKU: TRP050 Categories: ,

Description

The TriRNA Pure Kit is a spin column system for purification of high-quality total RNA from a variety of samples. Initially, samples are homogenized in TRIzol® Reagent, GENEzol™ Reagent or other phenol, guanidine isothiocyanate reagents. Chloroform phase separation or isopropanol RNA precipitation is not required. Following sample homogenization, simply bind, wash and elute the high-quality, total RNA in RNase-free Water and use in a variety of sensitive downstream applications.

Features & Benefits

  • Purify total RNA in 10 minutes!
  • No chlorophorm phase separation
  • No isopropanol RNA precipitation
  • No phenol carryover
  • Compatibility – homogenize samples with GENEzol™, TRI-Reagent®, TRIzol®, RNAzol®, QIAzol® etc.
  • Sample Volume – up to 200 μl of blood, buffy coat, serum, plasma), up to 5 x 106 cultured cells, 10-50 mg of tissue, 1 x 109 bacterial cells, 20-50 mg of plant tissue
  • A cost effective phenol, guanidine isothiocyanate solution plus spin column system
  • High quality RNA – A260/A280 >1.8, A260/A230 >1.8
  • Binding Capacity –  50 µg RNA from ≥ 25 µl DNase/RNase-free Water
  • Membrane – glass fibre membrane optimized for total RNA extraction
  • Spin Columns – individually packaged RNA spin columns, certified RNase and DNase-free

Applications

cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays, Northern Blotting

Contents

  • Pre-Wash Buffer
  • Wash Buffer
  • RNase-free Water
  • RB Columns
  • 2 ml Collection Tubes

Storage

Dry at room temperature – 15-25ºC

Protocol PDF

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