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Total RNA Mini Kit (Tissue) 50 Preps

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RT050 – Total RNA Mini Kit (Tissue) 50 Preps

SKU: RT050 Categories: ,

Description

The Total RNA Mini Kit (Tissue) is a RNA Isolation Kit designed for total RNA purification from a variety of animal tissue using an efficient RNA miniprep system. This RNA Kit will purify RNA from tissue samples within 15 minutes. Tissue samples can be efficiently homogenized in a microcentrifuge tube using the provided DNase and RNase-free Micropestle. The Total RNA Mini Kit (Tissue) uses detergents and chaotropic salt to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound by the glass fiber matrix of the RNA spin column. Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water. Phenol extraction or alcohol precipitation is not required and the purified RNA is ready for use in a variety of downstream applications.   Each Geneaid RNA Kit icludes individually packaged certified DNase and RNase-free spin columns and collection tubes.

Features & Benefits

    • Purify total RNA within 15 minutes
    • Sample – up to 25 mg of tissue  (tailsnips, liver, kidney, brain, spleen, earpunches etc)
    • High Yield – 5-30 μg of pure RNA
    • RNase and DNase-free Micropestles included for simple tissue homogenization within the tube to reduce the risk of sample contamination
    • Spin Columns – glass fibre membrane optimized for total RNA extraction
    • Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free

      Components

    • RB Buffer
    • W1 Buffer
    • Wash Buffer
    • RNase-free Water
    • RB Columns
    • Filter Columns
    • 2 ml Collection Tubes
    • Micropestles
    • RNA Mini Kit (Tissue) Functional Test Data

      Specifications
      Cat. #
      RB050
      RB100
      RB300
      RT050
      RT100
      RT300
      RP050
      RP100
      RP300
      RBB050
      RBB100
      RBB300
      RBY050
      RBY100
      RBY300
      RMI050
      GZX050
      GZX100
      GZX200
      Format
      Spin Column
      Spin Column
      Spin Column
      Spin Column
      Spin Column
      Spin Column
      Tri Reagent Spin Column
      Binding Capacity 50 µg 50 µg 50 µg 50 µg 50 µg 50 µg 50 µg
      Sample
      blood, cells
      animal tissue plant tissue bacterial cells yeast cells animal tissue, cells
      blood,cells,
      tissue, body fluids, bacteria
      Micropestle N/A Yes N/A N/A N/A Yes N/A
      Filter Column N/A Yes Yes N/A N/A N/A N/A
      Enzyme
      N/A
      N/A
      N/A
      Lysozyme
      N/A
      N/A
      N/A
      Typical Yield
      2-3 µg from

      300 µl blood,

      25-30 µg from
      106 cells
      5-30 µg
      5-30 µg
      40-50 µg from 1×109 bacterial cells
      15-20 µg from 5×107
      yeast cells
      5-30 µg
      5-30 µg
      Elution Volume 50 µl 50 µl 50 µl 50 µl 50 µl 50 µl 25-50 µl
      Time <20 min. <15 min. <20 min. <20 min. <20 min. <30 min. <15 min.

       

      Protocol PDF

      RB Buffer

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