Description
The Total RNA Mini Kit (Tissue) is a RNA Isolation Kit designed for total RNA purification from a variety of animal tissue using an efficient RNA miniprep system. This RNA Kit will purify RNA from tissue samples within 15 minutes. Tissue samples can be efficiently homogenized in a microcentrifuge tube using the provided DNase and RNase-free Micropestle. The Total RNA Mini Kit (Tissue) uses detergents and chaotropic salt to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound by the glass fiber matrix of the RNA spin column. Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water. Phenol extraction or alcohol precipitation is not required and the purified RNA is ready for use in a variety of downstream applications. Each Geneaid RNA Kit icludes individually packaged certified DNase and RNase-free spin columns and collection tubes.
Features & Benefits
- Purify total RNA within 15 minutes
- Sample – up to 25 mg of tissue (tailsnips, liver, kidney, brain, spleen, earpunches etc)
- High Yield – 5-30 μg of pure RNA
- RNase and DNase-free Micropestles included for simple tissue homogenization within the tube to reduce the risk of sample contamination
- Spin Columns – glass fibre membrane optimized for total RNA extraction
- Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
Components
- RB Buffer
- W1 Buffer
- Wash Buffer
- RNase-free Water
- RB Columns
- Filter Columns
- 2 ml Collection Tubes
- Micropestles
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RNA Mini Kit (Tissue) Functional Test Data
SpecificationsCat. # RB050RB100RB300RT050RT100RT300RP050RP100RP300RBB050RBB100RBB300RBY050RBY100RBY300RMI050GZX050GZX100GZX200FormatSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnTri Reagent Spin Column Binding Capacity 50 µg 50 µg 50 µg 50 µg 50 µg 50 µg 50 µg Sample blood, cellsanimal tissue plant tissue bacterial cells yeast cells animal tissue, cells blood,cells,tissue, body fluids, bacteriaMicropestle N/A Yes N/A N/A N/A Yes N/A Filter Column N/A Yes Yes N/A N/A N/A N/A Enzyme N/AN/AN/ALysozymeN/AN/AN/ATypical Yield2-3 µg from300 µl blood,
25-30 µg from106 cells5-30 µg5-30 µg40-50 µg from 1×109 bacterial cells15-20 µg from 5×107yeast cells5-30 µg5-30 µgElution Volume 50 µl 50 µl 50 µl 50 µl 50 µl 50 µl 25-50 µl Time <20 min. <15 min. <20 min. <20 min. <20 min. <30 min. <15 min. RB Buffer
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