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Total RNA Mini Kit (Tissue) 100 Preps

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RT100 – Total RNA Mini Kit (Tissue) 100 Preps

SKU: RT100 Categories: ,

Description

The Total RNA Mini Kit (Tissue) is a RNA Isolation Kit designed for total RNA purification from a variety of animal tissue using an efficient RNA miniprep system. This RNA Kit will purify RNA from tissue samples within 15 minutes. Tissue samples can be efficiently homogenized in a microcentrifuge tube using the provided DNase and RNase-free Micropestle. The Total RNA Mini Kit (Tissue) uses detergents and chaotropic salt to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound by the glass fiber matrix of the RNA spin column. Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water. Phenol extraction or alcohol precipitation is not required and the purified RNA is ready for use in a variety of downstream applications.   Each Geneaid RNA Kit icludes individually packaged certified DNase and RNase-free spin columns and collection tubes.

Features & Benefits

      • Purify total RNA within 15 minutes
      • Sample – up to 25 mg of tissue  (tailsnips, liver, kidney, brain, spleen, earpunches etc)
      • High Yield – 5-30 μg of pure RNA
      • RNase and DNase-free Micropestles included for simple tissue homogenization within the tube to reduce the risk of sample contamination
      • Spin Columns – glass fibre membrane optimized for total RNA extraction
      • Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free

        Components

      • RB Buffer
      • W1 Buffer
      • Wash Buffer
      • RNase-free Water
      • RB Columns
      • Filter Columns
      • 2 ml Collection Tubes
      • Micropestles

RNA Mini Kit (Tissue) Functional Test Data

Specifications
Cat. #
RB050
RB100
RB300
RT050
RT100
RT300
RP050
RP100
RP300
RBB050
RBB100
RBB300
RBY050
RBY100
RBY300
RMI050
GZX050
GZX100
GZX200
Format
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Tri Reagent Spin Column
Binding Capacity 50 µg 50 µg 50 µg 50 µg 50 µg 50 µg 50 µg
Sample
blood, cells
animal tissue plant tissue bacterial cells yeast cells animal tissue, cells
blood,cells,
tissue, body fluids, bacteria
Micropestle N/A Yes N/A N/A N/A Yes N/A
Filter Column N/A Yes Yes N/A N/A N/A N/A
Enzyme
N/A
N/A
N/A
Lysozyme
N/A
N/A
N/A
Typical Yield
2-3 µg from

300 µl blood,

25-30 µg from
106 cells
5-30 µg
5-30 µg
40-50 µg from 1×109 bacterial cells
15-20 µg from 5×107
yeast cells
5-30 µg
5-30 µg
Elution Volume 50 µl 50 µl 50 µl 50 µl 50 µl 50 µl 25-50 µl
Time <20 min. <15 min. <20 min. <20 min. <20 min. <30 min. <15 min.

 

Protocol Protocol PDF

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