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Total RNA Mini Kit (Plant) 50 Preps

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RP050 – Total RNA Mini Kit (Plant) 50 Preps

SKU: RP050 Categories: ,

Description

The Total RNA Mini Kit (Plant) is a RNA Isolation Kit designed for total RNA purification from plant tissue and cells using an efficient RNA miniprep system. This RNA Kit will purify RNA from a wide variety of plant species within 15 minutes. Samples are initially ground in liquid nitrogen and then filtered to remove cell debris using the efficient RNA filter columns. In the presence of a binding buffer and chaotropic salt, total RNA in the lysate binds to the glass fiber matrix of the RNA spin column. Once any contaminants have been removed using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water. Phenol extraction or alcohol precipitation is not required and the purified total RNA is ready for use in RT-PCR, Real-time PCR and Northern Blotting.   Each Geneaid RND Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes.

Features & Benefits

  • Purify total RNA within 15 minutes
  • High Yield –  5-30 μg of pure RNA
  • Sample Size – up to 100 mg (fresh plant tissue) and up to 25 mg (dry plant tissue)
  • Spin Columns – glass fibre membrane optimized for total RNA extraction
  • Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free

Contents

  • RB Buffer
  • PRB Buffer
  • W1 Buffer
  • Wash Buffer
  • RNase-free Water
  • Filter Columns
  • RB Columns
  • 2 ml Collection Tubes

Total RNA Mini Kit (Plant) Functional Test Data

Figure 1. Total RNA from 50 mg Thale Cress (Arabidopsis thaliana) leaves was extracted using the Total RNA Mini Kit (Plant). The purified RNA was analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA Ladder

Test Yield (μg) 260/280 260/230
Test 1 27.02 2.07 2.43
Test 2 27.68 2.10 2.40
Test 3 26.15 2.06 2.44

Total RNA Extraction Kits Specifications

Specifications
Cat. #
RB050
RB100
RB300
RT050
RT100
RT300
RP050
RP100
RP300
RBB050
RBB100
RBB300
RBY050
RBY100
RBY300
RMI050
GZX050
GZX100
GZX200
Format
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Tri Reagent Spin Column
Binding Capacity 50 µg 50 µg 50 µg 50 µg 50 µg 50 µg 50 µg
Sample
blood, cells
animal tissue plant tissue bacterial cells yeast cells animal tissue, cells
blood,cells,
tissue, body fluids, bacteria
Micropestle N/A Yes N/A N/A N/A Yes N/A
Filter Column N/A Yes Yes N/A N/A N/A N/A
Enzyme
N/A
N/A
N/A
Lysozyme
N/A
N/A
N/A
Typical Yield
2-3 µg from
300 µl blood,

25-30 µg from
106 cells
5-30 µg
5-30 µg
40-50 µg from 1×109 bacterial cells
15-20 µg from 5×107
yeast cells
5-30 µg
5-30 µg
Elution Volume 50 µl 50 µl 50 µl 50 µl 50 µl 50 µl 25-50 µl
Time <20 min. <15 min. <20 min. <20 min. <20 min. <30 min. <15 min.

 

Storage

Dry at room temperature (15 deg. C – 25 deg. C)

 

Protocol PDF

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