dnature diagnostics & research

Total RNA Mini Kit (Plant) 100 Preps

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RP100

SKU: RP100 Categories: ,

Description

The Total RNA Mini Kit (Plant) is a RNA Isolation Kit designed for total RNA purification from plant tissue and cells using an efficient RNA miniprep system. This RNA Kit will purify RNA from a wide variety of plant species within 15 minutes. Samples are initially ground in liquid nitrogen and then filtered to remove cell debris using the efficient RNA filter columns. In the presence of a binding buffer and chaotropic salt, total RNA in the lysate binds to the glass fiber matrix of the RNA spin column. Once any contaminants have been removed using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water. Phenol extraction or alcohol precipitation is not required and the purified total RNA is ready for use in RT-PCR, Real-time PCR and Northern Blotting. Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes.

Features & Benefits

  • Purify total RNA within 15 minutes
  • High Yield –  5-30 μg of pure RNA
  • Sample Size – up to 100 mg (fresh plant tissue) and up to 25 mg (dry plant tissue)
  • Spin Columns – glass fibre membrane optimized for total RNA extraction
  • Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
  • Storage: dry at room temperature (15-25°C)

Applications

RT-PCR, Northern Blotting, Real-time PCR

Components

  • RB Buffer
  • PRB Buffer
  • W1 Buffer
  • Wash Buffer
  • RNase-free Water
  • Filter Columns
  • RB Columns
  • 2 ml Collection Tubes

Protocol

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