Description

The RNA Kit (Blood/Cultured Cell) is a RNA Isolation Kit designed for total RNA purification from fresh whole human blood and cultured cells. This RNA Kit will purify RNA from blood within 20 minutes using an efficient RNA miniprep system. Detergents and chaotropic salt are used to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound by the glass fiber matrix of the RNA spin column and once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water and ready for use in various downstream applications. Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes.

Features & Benefits

• Purify total RNA within 20 minutes
• High Yield – up to 2µg from whole blood, up to 30µg from 1×10⁶ 293T cells
• Sample Volume – up to 300 µl of whole blood, cultured cells (up to 5 x 10⁶)
• Spin Columns: glass fibre membrane optimized for total RNA extraction
• Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free

Contents

• RBC Lysis Buffer
• RB Buffer
• W1 Buffer
• Wash Buffer
• RNase-free Water
• RB Columns
• 2ml Collection Tubes
  

  RNA Mini Kit (Blood/Cultured Cell) Functional Test Data

  	Figure 1. Total RNA from 1×106 293T cells was extracted using the Total RNA Mini Kit (Blood/Cultured Cell) and the equivalent competitors kit. The purified RNA was analyzed by electrophoresis on a 1% agarose gel. Lane 1, 2 = Geneaid, M = Geneaid 1 Kb DNA Ladder Lane 3, 4 = Competitor Geneaid Competitor Yield (μg) 260/280 260/230 Yield (μg) 260/280 260/230 28.70 1.96 2.32 20.50 1.85 2.31 29.90 1.99 2.02 28.90 1.84 2.32
  	Figure 2. Total RNA from 300 µl of whole human blood was extracted using the Total RNA Mini Kit (Blood/Cultured Cell) and the equivalent competitors kit. The purified RNA was analyzed by electrophoresis on a 1% agarose gel. Lane 1, 2 = Geneaid M = Geneaid 1 Kb DNA Ladder, Lane 3, 4 = Competitor Geneaid Competitor Yield (μg) 260/280 260/230 Yield (μg) 260/280 260/230 2.02 1.85 2.15 1.77 1.78 1.42 2.13 1.90 2.35 1.80 1.76 1.19
  Figure 2. Total RNA was purified from 101-103 HeLa cells using the Total RNA Mini Kit Blood/Cultured Cell (2 replications of each cell number). The purified RNA was eluted with 25 μl RNase-free Water. cDNA synthesis was carried out with a 10 μl aliquot of purified RNA (2/5) using a Transcriptor First Strand cDNA Synthesis Kit (Roche) in a final volume of 20 μl. A Real-time PCR assay was then performed with 8 µl of synthesized cDNA (2/5) as template, primers (designed to amplify human ACTB gene), and Fast SYBR Green PCR Master Mix using the StepOnePlusTM Real-Time PCR system (Applied Biosystems). The results confirmed that RNA can be successfully extracted and detected from as low as 101 HeLa cells. Legend 103 HeLa Cells, 102 HeLa Cells, 101 HeLa Cells

RNA Extraction Kit Specifications

Storage

Store dry at room temperature (15 deg. C – 25 deg. C)

Protocol PDF