Description

The GENEzol™ TriRNA Pure Kit is a phenol and guanidine isothiocyanate plus spin column system for convenient purification of high-quality total RNA from a variety of samples. Initially, samples are homogenized in GENEzol™ Reagent without chloroform phase separation or isopropanol RNA precipitation. Following sample homogenization, simply bind, wash and elute the high-quality, total RNA in RNase-free Water and use in a variety of sensitive downstream applications.

Features & Benefits

• Purify total RNA in 15 minutes!
• A cost effective phenol, guanidine isothiocyanate solution plus spin column system
• No chlorophorm phase separation
• No isopropanol RNA precipitation
• No phenol carryover
• Sample Volume: up to 200 μl of blood, buffy coat, serum, plasma, up to 5 x 10⁶ cultured cells, 10-50 mg of tissue, 1 x 10⁹ bacterial cells, 20-50 mg of plant tissue
• High quality RNA: A260/A280 >1.8, A260/A230 >1.8
• Binding Capacity: 50 µg RNA from ≥ 25 µl DNase/RNase-free Water
• Spin Columns: glass fiber membrane optimized for total RNA extraction (certified RNase and DNase-free)
• Storage: GENEzol™ Reagent should be stored at 2°C to 25°C, other components should be stored at room temperature (15-25°C)

Applications

Components

• GENEzol™ Reagent
• Pre-Wash Buffer
• Wash Buffer
• RNase-free Water
• RB Columns
• 2 ml Collection Tubes