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Geneaid™ DNA Isolation Kit (Bacteria) 1 ml/rxn

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Geneaid™ DNA Isolation Kit (Bacteria) 1 ml/rxn

SKU: GEE150 Categories: ,

Description

The Geneaid™ DNA Isolation Kit (Bacteria) offers a simple and gentle reagent DNA precipitation method for isolating high molecular weight genomic and viral DNA from Gram (+) positive and Gram (-) negative bacteria cells, suitable for archiving or sensitive downstream applications. This highly versatile solution based system can be scaled proportionately in order to satisfy larger sample volumes providing a convenient sample-storage procedure with minimal hands on time. Initially cells are lysed in the presence of detergents and a proprietary DNA stabilization solution followed by RNase A treatment. Once proteins and other contaminants are removed DNA is precipitated then rehydrated. The high quality extracted DNA is ready for use in a variety of downstream applications.

Features & Benefits

  • Isolate high molecular weight DNA for archiving or sensitive downstream applications
  • High yield, High quality DNA (A260/A280 = 1.8-2.0)
  • Sample: 0.5-1.5 x 109 bacteria cells
  • Easily scalable to satisfy larger bacteria sample volumes
  • Minimal hands on time
  • Convenient, simple and gentle reagent DNA precipitation method

Applications

DNA archiving, PCR, AFLP, RFLP/PADP, Southern Blotting, Real-time PCR

Contents

  • Cell Lysis Buffer
  • Gram+ Buffer
  • Protein Removal Buffer
  • DNA Hydration Buffer (10 mM Tris-HCL, 1 mM EDTA, pH8.0)

Storage

Dry at room temperature (15-25°C) for up to 2 years. Lysozyme is shipped at room temperature and should be stored at -20°C for up to 1 year

 

Protocol PDF

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