Description
- Minimal hands on time
- Convenient, simple and gentle reagent DNA precipitation method
Description
The Geneaid™ DNA Isolation Kit (Tissue) offers a simple and gentle reagent DNA precipitation method for isolating high molecular weight genomic, mitochondrial and viral DNA from tissue, suitable for archiving or sensitive downstream applications. This highly versatile solution based system can be scaled proportionately in order to satisfy larger sample volumes providing a convenient sample-storage procedure with minimal hands on time. Initially cells are lysed in the presence of detergents and a proprietary DNA stabilization solution followed by RNase A treatment. Once proteins and other contaminants are removed DNA is precipitated then rehydrated. The high quality extracted DNA is ready for use in a variety of downstream applications.
Contents
- Cell Lysis Buffer
- Protein Removal Buffer
- DNA Hydration Buffer (10 mM Tris-HCL, 1 mM EDTA, pH8.0)
- RNase A (10 mg/ml) – not included with GET1.5K
- Proteinase K
Storage
Store at dry room temperature (15-25 deg C) for up to 2 years. Proteinase K and RNase AN should be stored at -20 deg C for extended periods.
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