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Geneaid Bacteria DNA Isolation Kit, 100 preps

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GEE100 – Geneaid Bacteria DNA Isolation Kit, 100 preps

Description

The Geneaid™ DNA Isolation Kit (Bacteria) offers a simple and gentle reagent DNA precipitation method for isolating high molecular weight genomic and viral DNA from Gram (+) positive and Gram (-) negative bacteria cells, suitable for archiving or sensitive downstream applications. This highly versatile solution based system can be scaled proportionately in order to satisfy larger sample volumes providing a convenient sample-storage procedure with minimal hands on time. Initially cells are lysed in the presence of detergents and a proprietary DNA stabilization solution followed by RNase A treatment. Once proteins and other contaminants are removed DNA is precipitated then rehydrated. The high quality extracted DNA is ready for use in a variety of downstream applications.

Benefits

  • Isolate high molecular weight DNA for archiving or sensitive downstream applications
  • High yield, High quality DNA (A260/A280 = 1.8-2.0)
  • Sample: 0.5-1.5 x 109 bacteria cells
  • Easily scalable to satisfy larger bacteria sample volumes
  • Minimal hands on time
  • Convenient, simple and gentle reagent DNA precipitation method

Contents

  • Cell Lysis Buffer
  • Gram+ Buffer
  • Protein Removal Buffer
  • DNA Hydration Buffer (10 mM Tris-HCL, 1 mM EDTA, pH8.0)
  • RNase A (10mg/ml) – not included with GEE 1.5K
  • Lysozyme – not included with GEE1.5K

Genomic DNA Extraction Kit Specifications

Specifications
Cat. #
GEB100
GEB01K
GEB01K+
GEC150
GEC1.5K
GEC1.5K+
GET150
GET1.5K
GET1.5K+
GEE150
GEE1.5K
GEE1.5K+
GBY100
GBY300
GR100
GR200
RXNS 100-1,000 150-1,500 150-1,500 150-1,500 100-300 100-200
Format
Reagent System
Reagent System
Reagent System
Reagent System
Reagent System
Reagent System
Sample
300 µl-10 ml blood
up to 5×107 cultured cells
up to 200 mg tissue
up to 1.5×109 bacterial cells
up to 2×108 yeast cells
up to 1 g plant tissue
Elution Volume
100 µl-1 ml
50-200 µl
100-200 µl
100 µl
50-100 µl
50-100 µl
Operation Time
<40 minutes
<60 minutes
<70 minutes
<60 minutes
<40 minutes
<50 minutes

Storage

Dry at room temperature (15-25 deg C) for up to 2 years.  RNase A should be stored at 4 deg. C for extended periods.

Protocol

 

 

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