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Presto™ 96 Well Gel Extraction Kit 10X96 well plates/kit

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96DFG10 – Presto™ 96 Well Gel Extraction Kit 10X96 well plates/kit

SKU: 96DFG10 Categories: ,

Description

Protocol PDF

Presto™ 96 Well Gel Extraction Kits were designed to recover or concentrate DNA fragments from agarose gel. QG Buffer (yellow color indicating optimal pH≤7.5) is premixed with a pH indicator to ensure optimal pH, facilitate DNA binding and allow for easy observation of undissolved agarose gel. If pH exceeds the optimal level (>7.5), the color of the solution will appear purple instead of yellow. 3M Sodium Acetate (pH5.0) which is included with the kit, can then be added to the solution to adjust pH and return the color to yellow. Chaotropic salt is used to dissolve agarose gel and denature enzymes while DNA fragments are bound by the glass fiber matrix of the Presto™ Gel Extraction 96 Well Binding Plate. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. The pH indicator, salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation and the purified DNA is ready for use in subsequent reactions.

Features

  • Purify up to 300 mg of agarose gel slices per well in 45 minutes!
  • High Recovery: up to 80%
  • Format: Presto™ Gel Extraction 96 Well Binding Plates
  • Broad Fragment Size Range: 70 bp–20 kb
  • Centrifuge or vacuum manifold protocol
  • Elution Volume: 60 µl from 80 µl elution buffer volume, 40 µl from 60 µl elution buffer volume
  • Storage: dry at room temperature (15-25ºC) for up to 1 year without showing any reduction in performance

Applications

PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling and Ligation

Components

  • QG Buffer (premixed with pH indicator)
  • 3M Sodium Acetate (pH5.0)
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  • Presto™ Gel Extraction 96 Well Binding Plates
  • 0.35 ml Collection Plates
  • Adhesive Film

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