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RNase A solution (50 mg/ml) 50 µl

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RA500050

SKU: RA500050 Categories: , Tag:

Description

Ribonuclease A or RNase A is an endoribonuclease purified from bovine pancreas. RNase A is an important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, Ribonuclease protection assays, mapping single-base mutations in DNA/RNA. RNase A effectively cleaves the phosphodiester bond between the 5’-ribose of a nucleotide and the phosphate group attached to the 3’-ribose of an adjacent pyrimidine nucleotide which forms a 2’,3’-cyclic phosphate which is then hydrolyzed to the corresponding 3’-nucleoside phosphate.

Features & Benefits

  • Available in ready to use pre-mixed solutions: 50 μl (50 mg/ml), 130 μl (50 mg/ml), 200 μl (50 mg/ml), 1.5 ml (50 mg/ml), 550 µl (10 mg/ml), 1 ml (10 mg/ml)
  • RNase A solutions can be shipped at room temperature
  • Source: Bovine Pancreas
  • Molecular Weight: 13.7 kDa
  • Certified TSE/BSE Free
  • Storage: -20°C for extended periods, RNase A/ddH2O solution should be stored at 2-8°C for
    up to 6 months

Applications

Plasmid DNA purification, Genomic DNA purification, RNA removal from recombinant protein preparations, Ribonuclease protection assays, Mapping single-base mutations in DNA/RNA

Protocol PDF

 

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