PerfeCTa® DNase I is a high purity, recombinant bovine DNase I preparation that is free of any contaminating RNases or proteases. It provides a simple and rapid solution to eliminate residual genomic DNA from total RNA preparations for expression profiling by reverse transcription quantitative PCR amplification (RT-qPCR) as well as other molecular biology applications. The proprietary Reaction Buffer and Stop Buffer support a simple heat-kill step that permanently inactivates all trace levels of DNase activity. Complete inactivation of DNase I is critical before subsequent cDNA synthesis. Residual, or renatured, DNase will degrade cDNA product and thereby alter apparent expression levels.
One unit completely degrades 1µg of dsDNA in 10 minutes at 37°C.
Features & Benefits
- Permanent DNase I inactivation for confident first-strand cDNA synthesis with qScript® first-strand cDNA synthesis kits
- Single tube
- PerfeCta® DNase I (2U/µL, recombinant DNase I, RNase-free in, 50mM glycine (pH 7.2), 5mM calcium acetate, 50%(v/v) glycerol and stabilisers)
- 10X reaction buffer for DNase I
- 10X Stop Buffer
Shipping & Storage
PerfeCTa DNase I is stable for 2 years when stored in a constant temperature freezer at -20°C. The enzyme showed no loss in functional performance after 20 cycles of freezing on dry ice and thawing on ice. However, we recommend that the number of freeze-thaw cycles be kept to a minimum. For convenience, the 10X Reaction Buffer and 10X Stop Buffer may be stored at 2-8°C to avoid thawing the buffers for each use.