The High Sensitivity NGS Fragment Analysis Kit provides an automated solution to the quality assessment of low concentration NGS libraries prior to sequencing. The correct determination of molarity for optimum flow-cell loading is a critical step in NGS, and DNF-474 efficiently and accurately determines the average size of a library and its concentration. The correct calculation of molarity helps ensure quality NGS results. Use DNF-474 to increase the efficiency of your genomic research.
Features & Benefits
- Simplified sample handling – requires a single dilution step into a 96-well plate
- No chip loading – separation gel is automatically loaded into capillaries prior to each run
- High sensitivity – quantify library smears as low as 50pg/ul
- Short run times – complete analysis as quickly as 40 minutes
- Multi-Plate capacity – holds up to 288 samples in three, 96-well plates, sample rows/plates can be analysed in user-defined order
- Suitable for all NGS Platforms – provides quality and quantity analysis for the major NGS platforms
- NGS Fragment Separation Gel – 240ml
- Intercalating Dye – 30ul
- 5X 930 dsDNA Inlet Buffer – 125ml
- 5X Capillary Conditioning Solution – 50ml
- HS NGS Fragment Diluent Marker Solution – 2.4ml X 5 vials
- HS NGS Fragment DNA Ladder – 100ul
- 0.25X TE Rinse Buffer – 125ml
- BF-25 Blank Solution – 8ml
In the following section we have provided several examples of typical and atypical separations performed with the HS NGS Fragment Analysis Kit on a Fragment Analyzer fitted with a standard short array.
Figure 1 depicts a typical separation of the HS NGS DNA Ladder (DNF-396). The peaks are clearly defined and well separated, providing a precise and accurate standard curve for the sizing and quantitation of samples.
Assessment of the size and quality of a library smear is important to obtaining quality sequencing results. Figure 2 shows a high quality library constructed from commercially prepared human genomic DNA.
Importantly, the HS NGS Fragment Kit is able to detect primer dimers and adapter dimers, significant indicators of library quality. High levels of dimers, adapter or primer, interferes with sequencing, reducing the quality of the results. Figure 3 depicts a separation of an NGS library that has primer dimers and adapter dimers. Primer dimers typically appear around 70 bp, while adapter dimers appear around 140 bp.