The High Sensitivity Genomic DNA Analysis Kit is used for the automated analysis of low concentration genomic DNA in the 40 Kb range. Common applications include the evaluation of samples for next generation sequencing. It is also suited to the analysis of degraded DNA, such as FFPE preserved samples. Further streamlining workflows, the Genomic Quality Number (GQN) is calculated in PROSize®2.0 post-separation, providing a user-defined metric of sample quality. Note: This kit only uses a lower marker.
- Genomic DNA Separation Gel – 240ml
- Intercalating Dye – 30ul
- 5X 930 dsDNA Inlet Buffer – 125ml
- 5X Capillary Conditioning Solution – 50ml
- HS Genomic DNA Diluent Marker Solution – 2.4ml X 5 vials
- HS Genomic DNA ladder – 100ul
- 0.25X TE Rinse Buffer – 125ml
- BF-25 Blank Solution – 8ml
A typical separation of the High Sensitivity Genomic DNA Ladder (DNF-377) is shown in Figure 1. Electrophoresis was carried out on a Fragment Analyzer equipped with a Short Capillary Array (33-55) using the High Sensitivity Genomic DNA Analysis Kit (DNF-488). The clearly defined ladder peaks allow for the accurate measurement of genomic DNA samples, with an extended sizing range through 40,000 bp. The High Sensitivity Genomic DNA Analysis Kit (DNF-488) provides numerous advantages, including saving precious sample along with accurate and precise sizing through 40 Kb.
A separation of human gDNA with a Genomic Quality Number (GQN) of 9.6 is shown in Figure 2. Preparations of gDNA have a high degree of variation in quality for numerous reasons – including purification technique, age, and sample source – to the point that a fixed quality metric is of limited use. The GQN allows users to define what good quality gDNA is for their distinctive applications. The GQN is measured as the amount of sample that exceeds a user-selected size threshold, the resulting measurement reported on a scale of 0 to 10 with higher values corresponding to more of the sample falling above the defined threshold. The GQN changes with the selected threshold, if the user defines a threshold of 20,000 bp instead of the 10,000 bp threshold used in Figure 2, the GQN will be lower. An adaptive metric like the GQN provides users with more versatility in their analysis of diverse samples, as different downstream applications and sample sources have differing degrees of what qualifies as “good” gDNA.