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Honey DNA Extraction Kit (with lysing tubes), 100 Preps

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HDEB100

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Description

The Genomic DNA Honey Kit was designed for isolation of genomic DNA from pollen in small honey samples. PCR inhibitors are removed with a unique inhibitor removal buffer before mechanical lysis is used to efficiently lyse the sample material. Ceramic bead tubes are then used to efficiently homogenize the sample material. Following sample lysis, the flow-through is then mixed with a binding buffer and the genomic DNA is bound by the GS Column. The column is then washed and the DNA is eluted with Elution Buffer. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 60 minutes. The purified genomic DNA is ready for use in qPCR and other downstream applications.

Features & Benefits

  • Small scale – allows extraction from the smallest volumes of honey (1ml)
  • Novel inhibitory removal buffers
  • Familiar column based extraction
  • Includes bead beating tubes with optimised pre-filled bead mixtures
  • Designed for qPCR and NGS

Contents

  • PCR-Grade water
  • Pol Buffer
  • GT Buffer
  • GBT Buffer
  • W1 Buffer
  • Wash Buffer (add ethanol)
  • Proteinase K² (add ddH²O)
  • Elution Buffer
  • Beadbeating tubes Type D
  • GS Columns
  • 2ml Collection Tubes

Storage

Store at room temperature

extraction protocol

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