dnature diagnostics & research

Blog

Here you will find the musings of John.  Some of these were written almost a decade ago now but they remain relevant today.  We welcome your feedback.

Killing that DNA

By John Mackay | 08/11/2018

 You’ve done the hard work – you’ve got good quality RNA.  You’ve seen ribosomal bands on a gel (or *cough* Fragment Analyzer).  A good RQN/RIN (if that’s what you go by) and everything’s great. Now to get rid of the DNA Quick check (perform a PCR directly on appropriate amount of RNA) and often there’s […]

I have ‘what’ in my sample?

By John Mackay | 19/06/2017

Reagent contamination in PCR has long been documented – not only by operators but also from commercial sources long before those operators ever laid DNA-ridden hands on them. While contamination of some/many Taq polymerase enzymes with residual host E. coli DNA has had attention, little attention has been paid to the ubiquitous DNA extraction kits. […]

Killing that DNA

By John Mackay | 26/06/2016

You’ve done the hard work – you’ve got good quality RNA. You’ve seen ribosomal bands on a gel (or *cough* Fragment Analyzer). A good RQN/RIN (if that’s what you go by) and everything’s great. Now to get rid of the DNA Quick check (perform a PCR directly on appropriate amount of RNA) .. .and often […]