Designing primers for your high resolution melting is easy enough. A quick run with some known genotypes …. and look for the melting curve differences among samples. Too simple. But what about the temperature-neutral SNP’s? The what?
You’ve done the hard work – you’ve got good quality RNA. You’ve seen ribosomal bands on a gel (or *cough* Fragment Analyzer). A good RQN/RIN (if that’s what you go by) and everything’s great.
We should clarify; our work has “been done with the dogs”. Recently, dnature has taken the expertise that we have with DNA diagnostics and bee health, and teamed up with Rene Gloor and his dogs. Rene has trained
We’ve recently published an article in the Northern Farmer magazine as well as releasing our new bee virus testing ad. It’s important to have healthy hives over winter so that they come out “fighting fit” for the new honey
Reagent contamination in PCR has long been documented – not only by operators but also from commercial sources long before those operators ever laid DNA-ridden hands on them.
While contamination of some/many Taq polymerase enzymes with residual host E. coli
