dnature diagnostics & research

Touchdown PCR

Multiple reactions to run, each with their own preferred annealing temperature? Grabbed two primers of different Tm’s to amplify a region and trying to work out the best program?

Touchdown PCR to the rescue.

One of the best things to

Continue reading...


Designing primers for your high resolution melting is easy enough. A quick run with some known genotypes …. and look for the melting curve differences among samples. Too simple. But what about the temperature-neutral SNP’s? The what?

A number of

Continue reading...