Designing primers for your high resolution melting is easy enough. A quick run with some known genotypes …. and look for the melting curve differences among samples. Too simple. But what about the temperature-neutral SNP’s? The what?

A number of

1.  You’ve done the hard work – you’ve got good quality RNA.  You’ve seen ribosomal bands on a gel (or *cough* Fragment Analyzer).  A good RQN/RIN (if that’s what you go by) and everything’s great.
2. Now to get