OK, confession time. I used to work some crazy hours in order to fit some library screening into a part time job while finishing my degree. No, that’s not the confession – more, when I put the PCR on I
Designing primers for your high resolution melting is easy enough. A quick run with some known genotypes …. and look for the melting curve differences among samples. Too simple. But what about the temperature-neutral SNP’s? The what?