Getting the most off your PCR columns

Posted by John on 20 May 2010 | 0 Comments

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A quick tech tip I've found useful to maximise yield if using a low elution volume for column purification ie <50µl. I usually use this for PCR reaction purification but it also works for genomic DNA and probably other extractions as well (e.g. plasmid and RNA) if you're using lower elution volumes than suggested.

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