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Amplify them all and let the melting sort ‘em out.
OK perhaps my attitude to analysing sequence variation may be a little flippant but it characterises a lot of my thoughts toward High Resolution Melting Analysis (HRMA).
I put this tip out in our latest dnature newsletter. . .but couldn't find the reference to cite and thus included an offer to those who could use literature databases better than I.
However thanks to those who got in touch to say they already used the method (cheers Craig, Michael and Elena). I'm glad to know I didn't dream the thing.